pet21-b expression vector (Millipore)

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Millipore pet21-b expression vector
Pet21 B Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/the+pet21(b)+expression+vector/pet28a/pmc03055897-51-15-18
Average 90 stars, based on 1 article reviews

pet21-b expression vector - by Bioz Stars, 2026-09

90/100 stars

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Article Title: Endolysin OBPgpLYS
Article Snippet: .. Subsequently the cleaved nucleic acids encoding the peptide stretches were ligated into the pET21 b expression vector (Novagen, Darmstadt, Germany), which was also cleaved in a digest with the respective restriction enzymes Nde I and BamH I before. .. Afterwards, the nucleic acid molecule encoding the endolysin OBPgpLYS derivative was cleaved in a digest with the restriction enzyme BamH I and Xho I, so that the endolysin could be ligated into the pET21b expression vector (Novagen, Darmstadt, Germany).

Article Title: Antimicrobial agents
Article Snippet: .. Subsequently the cleaved nucleic acids encoding the peptide stretches were ligated into the pET21 b expression vector (Novagen, Darmstadt, Germany), which was also cleaved in a digest with the respective restriction enzymes Nde land BamH I before. .. The cleaved nucleic acid molecule encoding the peptide stretch Sushi I was ligated into a modified pET32 b expression vector (unmodified vector obtainable from Novagen, Darmstadt, Germany), which was also cleaved in a digest with the respective restriction enzymes Nco I and BamH I before.

Article Title: Composition for use in mycobacteria diagnosis
Article Snippet: .. Subsequently the cleaved nucleic acids encoding the peptide stretches were ligated into the pET21 b expression vector (Novagen, Darmstadt, Germany), which was also cleaved in a digestion with the respective restriction enzymes Nde I and BamH I before. .. The cleaved nucleic acid molecule encoding the peptide stretch and PTD for ligation with toxic proteins was ligated into a modified pET32 b expression vector (unmodified vector obtainable from Novagen, Darmstadt, Germany), which was also cleaved in a digestion with the respective restriction enzymes Nco I and BamH I before.

Article Title: Modified KZ144 endolysin sequence
Article Snippet: .. Subsequently the cleaved nucleic acids encoding the peptide stretch was ligated into the pET21 b expression vector (Novagen, Darmstadt, Germany), which was also cleaved in a digest with the respective restriction enzymes NdeI and BamHI before. .. Afterwards, the nucleic acid molecule encoding the endolysin was cleaved in a digest with the restriction enzyme BamHI and XhoI, so that the endolysin could be ligated into the pET21b expression vector (Novagen, Darmstadt, Germany), which was also cleaved in a digest with the respective restriction enzymes BamHI and XhoI before.

other:

Article Title: Signal peptide of FadA adhesin from Fusobacterium nucleatum plays a novel structural role by regulating the filament's length and width
Article Snippet: 2.1-Bacterial strains and cell lines E. coli BL21(DE3)-pYWH417-6 carrying the entire fadA gene in the pET21(b) expression vector (Novagen) with a His-tag fusion at the carboxyl terminal was used to produce FadAc ( 13 ).

Article Title: The Human Metapneumovirus Matrix Protein Stimulates the Inflammatory Immune Response In Vitro
Article Snippet: The Nhe I and Bam HI (underlined) digested PCR products were ligated into the digested pET21-b expression vector (Novagen).

Article Title: Signal peptide of FadA adhesin from Fusobacterium nucleatum plays a novel structural role by regulating the filament's length and width
Article Snippet: E. coli BL21(DE3)-pYWH417-6 carrying the entire fadA gene in the pET21(b) expression vector (Novagen) with a His-tag fusion at the carboxyl terminal was used to produce FadAc ( 13 ).

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